Презентация «Cellulase. Introduction (source)»
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Cellulase
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Introduction (source) Cellulase refers to an entourage of enzymes produced chiefly by fungi, bacteria and protozoans that catalyze cellulolysis (i. e. the hydrolysis of cellulose).
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Cellulase
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Some species of fungi and bacteria are able to exhaustively digest crystalline cellulose in pure culture are said to have complete or true cellulases. The majority of organisms that produce …
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Other Names Other names for 'endoglucanases' are: endo-1,4-beta-glucanase, carboxymethyl cellulase (CMCase), endo-1,4-beta-D-glucanase, beta-1,4-glucanase, beta-1,4-endoglucan hydrolase, …
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Types of reactions/ Classification General types of cellulases based on the type of reaction catalyzed: Cleaves internal bonds at Endocellulase (EC 3. 2. 1. 4) randomly amorphous sites that create …
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Choice of host organism
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Strain engineering Thermostable cellulases production Nowadays, most of the studies about production of thermostable cellulases are focused on the utilization of cellulase-producing …
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Homologous overexpression in bacteria Some studies report the use of directed evolution techniques in combination with a rational design to overexpress cellulases in their own bacterial source. …
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Heterologous overexpression The strategies based in heterologous expression are focused in the use of non-cellulolytic micro/organisms that have high production ratio for expressing microbial …
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Future targets for genetic manipulation and optimization will include the use of the cellulolytic system of Clostridium thermocellum for engineering new strains, depending of the concrete industrial …
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Cultivation Media
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Harvest and Separation of Enzymes
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Uses
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